Suspension of hydrophobic microspheres in water (aqueous) solution, specifically for flow visualization, Particle Image Velocimetry (PIV) or biological applications require the use of a surfactant.
For non bio-sensitive
industrial flow visualization applications
We recommend to use concentrated Simple Green All Purpose Cleaner (www.simplegreen.com) Cover dry spheres with concentrated simple green
and let sit for approximately 5 min before dispersing in water.
For biocompatible aqueous dispersion applications:
Refer to Protocol for Preparing Tween Solutions of Hydrophobic Polyethylene Microspheres.